Skup podataka
Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''
Institut Ruđer Bošković, 2024. urn:nbn:hr:241:762376
Popović, Marta; Antičević, Ivan; Otten, Cecile

 Skup podataka
Skup podataka: figure_2_rtpcr_data.zip, 466.8 KB
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Opis datoteke:
The RTPCR data corresponding to Figure2C and D in the manuscript. (engleski)

Skup podataka: figure_2b_rtpcr_embryonicexpression.zip, 25.69 KB
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Opis datoteke:
The RTPCR data corresponding to Figure2B in the manuscript. (engleski)

Skup podataka: figure_3_s3_enzymatic_activity.zip, 11.43 MB
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Opis datoteke:
The enzymatic activity data corresponding to Figures 3 and S3 in the manuscript. (engleski)

Skup podataka: figure_3d_embryo_images.zip, 451.91 KB
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Opis datoteke:
The phenotype (images) corresponding to Figure 3D in the manuscript. (engleski)

Skup podataka: figure_4_totaldpc_data.zip, 6.38 MB
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Opis datoteke:
The DNA-protein crosslink data corresponding to Figure 4 in the manuscript. (engleski)

Skup podataka: figure_5_dpc_gh2ax_data.zip, 8.13 MB
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Opis datoteke:
The DNA-protein crosslink and gH2AX data corresponding to Figure 5 in the manuscript. (engleski)

Skup podataka: figure_s2c_rtpcr_data.zip, 235.38 KB
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Opis datoteke:
The RTPCR data corresponding to Figure S2C in the manuscript. (engleski)

Skup podataka: figure_s4b_embryo_images.zip, 411.61 KB
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Opis datoteke:
The phenotype data (images) corresponding to Figure S4B in the manuscript. (engleski)

Skup podataka: figure_s5a_embryo_images.zip, 2.35 MB
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Opis datoteke:
The phenotype data (images) corresponding to Figure S5A in the manuscript. (engleski)
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Popović, M., Antičević, I. i Otten, C. (2024). Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'' [Skup podataka]. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, Marta, et al. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković, 2024. 19.11.2024. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, Marta, Ivan Antičević, i Cecile Otten. 2024. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, M., Antičević, I. i Otten, C. 2024. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković. [Online]. [Citirano 19.11.2024.]. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović M, Antičević I, Otten C. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. [Internet]. Institut Ruđer Bošković: Zagreb, Bijenicka cesta 54, HR; 2024, [pristupljeno 19.11.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:241:762376.

M. Popović, I. Antičević i C. Otten, Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'', Institut Ruđer Bošković, 2024. Citirano: 19.11.2024. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:241:762376.

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:241:762376
Naslov (engleski)Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''
AutorMarta Popović
Ruder Boskovic institute
AutorIvan Antičević
Ruder Boskovic institute
AutorCecile Otten
Ruder Boskovic institute
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Biologija
Biokemija i molekularna biologija
Sažetak (engleski)
The raw experimental data which was analysed and published in the manuscript '' Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'' is uploaded in this repository. The raw data include enzymatic actvity data in .xlsx, .pzfx, and .tif. RTPCR results in .xlsx and .pzfx; images of zebrafish embryos in .jpg and .tif; visualization and quantification of DNA-protein crosslinks and γH2AX western blots in .xlsx, .pzfx and .tif.
Metodologija (engleski)DPC isolation was performed using modified RADAR (rapid approach to DNA adduct recovery) assay. DPCs were separated using SDS-PAGE electrophoresis and visualized using silver staining. Specific DPCs were visualized using dot blot analysis and detected with specific antibodies. yH2AX levels were visaulized using Western blot. Quantification of silver-stained gels, Dot blots and Western blots was conducted using the ImageJ software. Zebrafish embryos were imaged using a Samsung 13-megapixel camera with an f/1.9 aperture applied to the ocular of Motic SMZ-171 binocular. RTPCR was used to determine gene expression in zebrafish embryos and adults. Graphical representation of the expression data and statistical analysis were conducted using the unpaired two-tailed Student's t-test with GraphPad Prism 8 software.
Ključne riječi (engleski)
Jezikengleski
URN:NBNurn:nbn:hr:241:762376
Datum objave2024-07-15
GeolokacijaZagreb, Bijenicka cesta 54, Hrvatska
ProjektŠifra: UIP-2017-05-5258
Naziv (engleski): Deciphering DNA-Protein Crosslink Repair in vivo using zebrafish model
Kratica: DNAPRO
Voditelj: Marta Popović
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Vanjski povezani objektCitiran od: DOI 10.3389/fcell.2024.1394531
IzdavačInstitut Ruđer Bošković
Ruđer Bošković Institute
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Prenošenje u javno dobro (CC0 1.0)
Datum i vrijeme pohrane2024-06-21 09:55:23