Dataset
Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''
Ruđer Bošković Institute, 2024. urn:nbn:hr:241:762376
Popović, Marta; Antičević, Ivan; Otten, Cecile

 Dataset
Dataset: figure_2_rtpcr_data.zip, 466.8 KB
Access Condition: Open access
Description:
The RTPCR data corresponding to Figure2C and D in the manuscript. (English)

Dataset: figure_2b_rtpcr_embryonicexpression.zip, 25.69 KB
Access Condition: Open access
Description:
The RTPCR data corresponding to Figure2B in the manuscript. (English)

Dataset: figure_3_s3_enzymatic_activity.zip, 11.43 MB
Access Condition: Open access
Description:
The enzymatic activity data corresponding to Figures 3 and S3 in the manuscript. (English)

Dataset: figure_3d_embryo_images.zip, 451.91 KB
Access Condition: Open access
Description:
The phenotype (images) corresponding to Figure 3D in the manuscript. (English)

Dataset: figure_4_totaldpc_data.zip, 6.38 MB
Access Condition: Open access
Description:
The DNA-protein crosslink data corresponding to Figure 4 in the manuscript. (English)

Dataset: figure_5_dpc_gh2ax_data.zip, 8.13 MB
Access Condition: Open access
Description:
The DNA-protein crosslink and gH2AX data corresponding to Figure 5 in the manuscript. (English)

Dataset: figure_s2c_rtpcr_data.zip, 235.38 KB
Access Condition: Open access
Description:
The RTPCR data corresponding to Figure S2C in the manuscript. (English)

Dataset: figure_s4b_embryo_images.zip, 411.61 KB
Access Condition: Open access
Description:
The phenotype data (images) corresponding to Figure S4B in the manuscript. (English)

Dataset: figure_s5a_embryo_images.zip, 2.35 MB
Access Condition: Open access
Description:
The phenotype data (images) corresponding to Figure S5A in the manuscript. (English)
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Cite this document

Popović, M., Antičević, I. & Otten, C. (2024). Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'' [Data set]. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, Marta, et al. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković, 2024. 28 Aug 2024. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, Marta, Ivan Antičević, and Cecile Otten. 2024. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković. https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović, M., Antičević, I. and Otten, C. 2024. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. Institut Ruđer Bošković. [Online]. [Accessed 28 August 2024]. Available from: https://urn.nsk.hr/urn:nbn:hr:241:762376.

Popović M, Antičević I, Otten C. Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''. [Internet]. Institut Ruđer Bošković: Zagreb, Bijenicka cesta 54, HR; 2024, [cited 2024 August 28] Available from: https://urn.nsk.hr/urn:nbn:hr:241:762376.

M. Popović, I. Antičević and C. Otten, Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'', Institut Ruđer Bošković, 2024. Accessed on: Aug 28, 2024. Available: https://urn.nsk.hr/urn:nbn:hr:241:762376.

Title (english)Raw data from the publication ''Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo''
AuthorMarta Popović
Ruder Boskovic institute
AuthorIvan Antičević
Ruder Boskovic institute
AuthorCecile Otten
Ruder Boskovic institute
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Biochemistry and Molecular Biology
Abstract (english)
The raw experimental data which was analysed and published in the manuscript '' Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo'' is uploaded in this repository. The raw data include enzymatic actvity data in .xlsx, .pzfx, and .tif. RTPCR results in .xlsx and .pzfx; images of zebrafish embryos in .jpg and .tif; visualization and quantification of DNA-protein crosslinks and γH2AX western blots in .xlsx, .pzfx and .tif.
Methods (english)DPC isolation was performed using modified RADAR (rapid approach to DNA adduct recovery) assay. DPCs were separated using SDS-PAGE electrophoresis and visualized using silver staining. Specific DPCs were visualized using dot blot analysis and detected with specific antibodies. yH2AX levels were visaulized using Western blot. Quantification of silver-stained gels, Dot blots and Western blots was conducted using the ImageJ software. Zebrafish embryos were imaged using a Samsung 13-megapixel camera with an f/1.9 aperture applied to the ocular of Motic SMZ-171 binocular. RTPCR was used to determine gene expression in zebrafish embryos and adults. Graphical representation of the expression data and statistical analysis were conducted using the unpaired two-tailed Student's t-test with GraphPad Prism 8 software.
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:241:762376
Publication2024-07-15
GeolocationZagreb, Bijenicka cesta 54, Croatia
ProjectNumber: UIP-2017-05-5258
Title (english): Deciphering DNA-Protein Crosslink Repair in vivo using zebrafish model
Acronym: DNAPRO
Leader: Marta Popović
Jurisdiction: Croatia
External related objectIs cited by: DOI 10.3389/fcell.2024.1394531
PublisherInstitut Ruđer Bošković
Ruđer Bošković Institute
Access conditionsOpen access
Terms of useLicence Public Domain Dedication icon
Created on2024-06-21 09:55:23