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Barac, M., Siketić, Z., Bogdanović Radović, I. & Brajković, M. (2021). Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type [Data set]. doi:10.5281/zenodo.5957798
Barac, Marko, et al. Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type. Institut Ruđer Bošković, 2021. 14 Nov 2024. doi:10.5281/zenodo.5957798
Barac, Marko, Zdravko Siketić, Ivančica Bogdanović Radović, and Marko Brajković. 2021. Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type. Institut Ruđer Bošković. doi:10.5281/zenodo.5957798
Barac, M., et al. 2021. Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type. Institut Ruđer Bošković. [Online]. [Accessed 14 November 2024]. Available from: https://doi.org/10.5281/zenodo.5957798
Barac M, Siketić Z, Bogdanović Radović I, Brajković M. Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type. [Internet]. Institut Ruđer Bošković: Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, HR; 2021, [cited 2024 November 14] Available from: https://doi.org/10.5281/zenodo.5957798
M. Barac, Z. Siketić, I. Bogdanović Radović and M. Brajković, Spatially resolved metabolic composition in seeds of common bean: comparison of the low phytic acid mutant and the wild type, Institut Ruđer Bošković, 2021. Accessed on: Nov 14, 2024. Available: https://doi.org/10.5281/zenodo.5957798
Paula Pongrac (Researcher) Jožef Stefan Institute, Ljubljana, Slovenia
Collaborator
Katarina Vogel-Mikuš (Researcher) Jožef Stefan Institute, Ljubljana, Slovenia
Scientific / art field, discipline and subdiscipline
NATURAL SCIENCES Biology Botany
Abstract (english)
Common bean (Phaseolus vulgaris L.) seeds are a good source of energy, are rich in proteins and carbohydrates, minerals and vitamins (such as Fe, Zn, B-vitamin), and bioactive compounds, such as polyphenols. However, the presence of some antinutritional compounds, such as phytic acid (PA), which decreases mineral bioavailability, can limit the nutritional value of common beans. Therefore, genotypes with low PA concentrations in common beans have been generated. The increased bioavailability of Fe from LPA mutant seeds compared to the wild type common beans was shown in a stable Fe-isotope absorption study in Swiss women, indicating that the seeds of LPA common bean could be used to help remedy the Fe malnutrition in women. Within this TNA project, we spatially resolved molecular composition in LPA mutant and wild-type common beans, particularly the distribution of PA. In total, three replicates of each genotype were analyzed with MeV-SIMS at RBI. Positive and negative modes were operated for analysis of the samples and of the standard (PA). Best spectra were obtained in negative mode, in which three distinct peaks were observed in the standard (PA): 63 m/z: PO2-, 79 m/z: PO3- and 97 m/z: H2PO4-.
Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia
Geolocation
Jožef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
Project
Number: 824096 Title (english): Research And Development with Ion Beams – Advancing Technology in Europe Acronym: RADIATE Leader: Iva Bogdanović Radović Jurisdiction: eu Funding stream: H2020